Native MS

Native Mass Spectrometry has emerged as standard tool in characterizing the structure and dynamics of biomolecular complexes. It can provide insights in the stoichiometry and topology of subunits by inducing partial dissociation of the protein complex.

Since 2005 MS Vision has been active in the field of Native Mass Spectrometry and has fulfilled a pioneering role in the development of the technique. In close collaboration with the group of prof. Albert Heck at Utrecht University, we have gradually and steadily improved the technology for the analysis of intact protein complexes, often non-covalently bound, preserving native conditions in the gas phase.

As a small, dedicated and focused company, we have developed our technology through modification of Waters series Q-Tof and LCT instruments in response to specific user feedback. We moved on to translate what we had learnt to Synapt instruments, yielding excellent high mass performance and turning  Synapt instruments into versatile platforms for structural biochemistry. Our High Mass Synapt systems comprise:

  • Extension of the quadrupole m/z range to 32 kDa, enabling efficient transmission and selection of large protein complexes
  • Enhanced control over ion source and transfer region conditions, allowing for softer operation for better preservation of fragile proteins, and hotter conditions for enhanced activation of stable complexes
  • Enhanced activation and collisional cooling control in the TriWave region, allowing the admission of different gases into the IMS stage so it can either be used for post-activation cooling or Ion Mobility.
  • Customized acquisition settings, for best signal-to-noise in real-time monitoring
  • Preserving the Ion Mobility capability of the instrument

Applications range from the analysis of antibodies (Mw determination and purity) Antigen-Antibody interaction studies (stochiometry and affinity studies); Antibody PTM analysis (glycosilation, Antibody Drug Conjugates (ADC)); protein-protein and protein-ligand interactions (stochiometry, topology ) and virus capsids (composition, viral drugs development).

Thank you for delivering such a nice instrument!

I would like to thank you again for your efforts in setting up our High Mass Q-Tof-MS and in introducing my students to the basic operating principles of the instrument. We performed the first measurements this week, which went really well. You did a great job and we are very happy with our new toy.

Prof. Dr. Andrea Sinz
Martin-Luther University Halle-Wittenberg

Thank you for the repair and training.

I would like to take the opportunity to thank you for the repair of our Quattro Micro and subsequent ‘hands on’ training course. The training course was very useful, generating positive feedbacks from the attendees. We look forward to receiving the training course certificates.

Dr. Nancy Weisbach
Across Barriers GmbH

MS Vision is our long lasting services partner

MS Vision is our long lasting services partner for the maintenance and troubleshooting of our entire fleet of LC-MS/MS instruments. For us, their eagerness to extend their portfolio with new services and collaboratively identify ways of improvements is very much appreciated and fundamental in the reduction of instrument downtime that has been achieved.

Niels Martha
TLR International Laboratories

Installation of five Thermo mass spectrometers at Biomedicum, Karolinska Institutet, Stockholm

The team of MS Vision has recently helped us to relocate our entire mass spectrometry lab, including five Orbitrap instruments (three Q Exactive series, one Elite and one Fusion) within the campus of Karolinska Institutet. Both the preparation and execution of this delicate operation was performed with absolute profession and accuracy. After the careful move, it took only two days to get all systems up and running like they would have never been down for a second. Needless to say how grateful we are for MS Vision for this excellent job!

Akos Vegvari, PhD
Proteomics Biomedicum, Karolinska Institutet

“I love how MS Vision makes us better!“

Boris Krichel

Thank you for your cleaning effort on our Sciex 5500

Since last Wednesday we ran around 100 samples, everything is perfect (better sensitivity and linear dynamic range).  I have checked some data back to the last October, since I know what I am looking for, it seems that the charging problems started around December/January. Next time I will realize it much earlier.  Thank you both for your cleaning effort 😉


Manuel Mazenauer
MSVision Dedicated to Mass Spectrometry

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