Challenging the mass spectrometer

Overcoming the limitations of standard MS instrumentation in the analysis of large protein structures has been the focus of our efforts over the past decade. By working together closely with leading academic groups, we learnt how to get the best out of the mass spectrometer. The result is an instrument which allows researchers to unleash the full capacity of the Synapt in the analysis of intact proteins and complexes by native electrospray mass spectrometry.

Mass spectrum of empty and loaded adeno-associated virus particles
An ion source on a mass spectrometry instrument

Turning the Synapt into a Native Electrospray MS platform

The interest in MS of large biomolecules and biomolecular complexes has increased in recent years. Mass spectrometric study of intact proteins and their function in non-covalently bound complexes is providing more information about complicated biological systems. It is well known that intact large protein complexes can be transferred into the gas phase using nano-electrospray ionisation under native conditions, but subsequent MS or MS/MS analysis is prohibited by mass-scale, ion transmission or ion thermalisation limitations on standard instrumentation. These limitations have now been overcome, and all analytical tools available on the Synapt can be fully exploited in the study and analysis or large proteins and their complexes.

Making it work better

By careful consideration of the conditions that exist in the consecutive sections of the instrument, the performance of the Synapt for large ions can be improved considerably by changing the characteristics of the ion optical elements

No compromise

All functionality of the original instrument is available on the Native Synapt. Simply choose between modes and analyze your samples. Soft conditions for preservation of complexes, high energy dissociation, Ion Mobility – it’s all possible!

Upgrade or full instrument

Whether you want to have your own Synapt upgraded or need a complete instrument, MS Vision has the solution. We offer full support by our highly skilled engineers so you can concentrate on running your samples.

Excellent service, as always. Many thanks Alex!

Anonymous from feedback form after LTQ-Orbitrap service

I would like to thank you again for your efforts in setting up our High Mass Q-Tof-MS and in introducing my students to the basic operating principles of the instrument. We performed the first measurements this week, which went really well. You did a great job and we are very happy with our new toy.

Prof. Dr. Andrea Sinz
Martin-Luther University Halle-Wittenberg

I would like to take the opportunity to thank you for the repair of our Quattro Micro and subsequent ‘hands on’ training course. The training course was very useful, generating positive feedbacks from the attendees. We look forward to receiving the training course certificates.

Dr. Nancy Weisbach
Across Barriers GmbH

The team of MS Vision has recently helped us to relocate our entire mass spectrometry lab, including five Orbitrap instruments (three Q Exactive series, one Elite and one Fusion) within the campus of Karolinska Institutet. Both the preparation and execution of this delicate operation was performed with absolute profession and accuracy. After the careful move, it took only two days to get all systems up and running like they would have never been down for a second. Needless to say how grateful we are for MS Vision for this excellent job!

Akos Vegvari, PhD
Proteomics Biomedicum, Karolinska Institutet

MS Vision is our long lasting services partner for the maintenance and troubleshooting of our entire fleet of LC-MS/MS instruments. For us, their eagerness to extend their portfolio with new services and collaboratively identify ways of improvements is very much appreciated and fundamental in the reduction of instrument downtime that has been achieved.

Niels Martha
TLR International Laboratories

Very friendly and competent. We got a few new tips for our cleaning routine.

Anonymous from feedback form after Sciex API4000 maintenance
LMU Munich

“I love how MS Vision makes us better!“

Boris Krichel
Hamburg

Excellent and very competent service as always. We are very satisfied. Thanks again that you made it possible at such short notice.

Anonymous from feedback form after Waters Synapt maintenance
University of Freiburg

The highly skilled service technicians of MS Vision are a keystone for us to achieve the highest uptime possible.

Dominic Helm
EMBL

We are super happy that Jerre could fix our high mass QTOF II! It was a big task this time, but now I am confident we will be able to get good data with the instrument for many more years! The service is absolutely fantastic!!!

Anonymous from feedback form after High mass QTOF2 repair
Martin-Luther-Universität Halle-Wittenberg

Great Job done!

Anonymous from feedback form after Waters QTOF Ultima maintenance

Very happy with the professional survey of and repairs performed on our XEVO G2XS Q-ToF!

Anonymous from feedback form after Xevo QTOF G2-XS repair

Since last Wednesday we ran around 100 samples, everything is perfect (better sensitivity and linear dynamic range).  I have checked some data back to the last October, since I know what I am looking for, it seems that the charging problems started around December/January. Next time I will realize it much earlier.  Thank you both for your cleaning effort 😉

 

Manuel Mazenauer

Perfect service!

Anonymous from feedback form after LTQ-Orbitrap XL relocation
Martin-Luther-Universität Halle-Wittenberg