We will likely have several refurbished Thermo QExactive HF-X systems available for sale in early 2025! Contact us if you are interested!

The systems are currently located at different customer sites in Germany  and will become available early 2025. We strongly recommend to operate the systems under high flow conditions with at least 800µm-1mm column diameter. When being operated using nanoLC, the system suffers from fast contamination and has to  be cleaned frequently. Using cap/microLC conditions it can be operated very stable under high-throughput conditions.

Understanding biomolecules as drug targets, disease markers, and therapeutic agents demands highly sensitive analysis of complex biological samples, without sacrificing robustness and speed. The Thermo Q-Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer rises to the challenge as a versatile platform, setting new standards in sensitivity, performance and productivity for any workflow. Building on the proven performance of the Thermo QExactive hybrid quadrupole-Orbitrap MS family of instruments, the new Q-Exactive HF-X mass spectrometer couples a high field Thermo Scientific Orbitrap mass analyzer with a high capacity transfer tube and electrodynamic ion funnel (EDIF) that maximize ion loading and transmit ions over a broad mass range.

As a result, the instrument delivers a bright, focused and ion-rich beam, which translates to high sensitivity and excellent stability in analysis quality from run to run. Smart instrument control features match the superior ion transmission with increased acquisition speed and even more sophisticated data-dependent acquisition and analysis strategies to meet data quality needs at every stage of your work, from discovery to verification and quantitation.

Features:

• Novel architecture with a high capacity transfer tube (HCTT) and electrodynamic ion funnel that increases ion transmission and boosts sensitivity for all analytes, from small molecules to native antibodies.
• Increased acquisition speed and advanced precursor determination (APD) dramatically improves peptide sequencing speed and depth.
• Robust quantitative workflows, including parallel reaction monitoring (PRM) and high-resolution data-independent acquisition (HR-DIA).
• The Advanced Active Beam Guide (AABG) reduces noise and improves instrument robustness

Further information:

• Thermo QExactive HF-X specifications
• Thermo Q Exactive HF-X brochure
• Thermo Q-Exactive HF-X site prep requirements